Journal: Pharmaceuticals

Article Title: UHPLC–Q–Orbitrap–HRMS-Based Multilayer Mapping of the Pharmacodynamic Substance Basis and Mechanistic Landscape of Maizibizi Wan in Chronic Nonbacterial Prostatitis Therapy

doi: 10.3390/ph19010153

Figure Lengend Snippet: MZBZ regulates key target protein expression. Western blot image of ( A ) P-p65/p65, ( B ) p-AKT/AKT, ( C ) BCL2, COX-2, and TGF-β1 protein expression.

Article Snippet: Antibodies and kits included a BCA protein assay kit (Cat. No. WLA004, Wanleibio, Shenyang, China) and primary antibodies against p-NF-κB p65 (WL02169), NF-κB p65 (WL01980), BCL2 (WL01556), COX-2 (WL01750), AKT1 (WL01652), p-AKT (WLP001), TGF-β1 (WL02193), and β-actin (WL01372), together with HRP-conjugated goat anti-rabbit IgG (WLA023; all from Wanleibio, Shenyang, Chnia).

Techniques: Expressing, Western Blot

Journal: PLOS One

Article Title: Inhibition of TREM1 attenuates myocardial ischemia-reperfusion injury-induced cardiomyocyte pyroptosis by suppressing the activation of the NF-κB signaling pathway

doi: 10.1371/journal.pone.0340382

Figure Lengend Snippet: The (A-C) WB method was used to detect the expression of NF-κB signaling pathway related proteins p-IkBa, IkBa, p-NF-κB, and NF-κB. (D-E) Confocal microscopy for observing NF-κB nuclear translocation. (scale bar: 20 μm). (F-G) NLRP3 fluorescence assay for observing NLRP3 expression in different groups. (scale bar: 100 μm).ns indicates no statistical significance,*P < 0.05,**P < 0.01.

Article Snippet: Primary antibody against NF-κB p65 (Abmart, T55034F) was added at a dilution of 1:100 and incubated at 4°C overnight.

Techniques: Expressing, Confocal Microscopy, Translocation Assay, Fluorescence

Journal: PLOS One

Article Title: Inhibition of TREM1 attenuates myocardial ischemia-reperfusion injury-induced cardiomyocyte pyroptosis by suppressing the activation of the NF-κB signaling pathway

doi: 10.1371/journal.pone.0340382

Figure Lengend Snippet: (A-H) WB method was used to detect the expression of NF-κB signaling pathway related proteins p-IkBa, IkBa, p-NF-κB, and NF-κB and the expression of pyroptosis related proteins NLRP3、GSDMD-N、 Cleaved-Caspase-1, and mature IL-1β (IL-1β)、mature IL-18. (I-N) WB method was used to detect the expression of pyroptosis-related proteins after inhibiting NLRP3 with MCC950.ns indicates no statistical significance,*P < 0.05,**P < 0.01,***P < 0.001.

Article Snippet: Primary antibody against NF-κB p65 (Abmart, T55034F) was added at a dilution of 1:100 and incubated at 4°C overnight.

Techniques: Expressing

Journal: PLOS One

Article Title: Inhibition of TREM1 attenuates myocardial ischemia-reperfusion injury-induced cardiomyocyte pyroptosis by suppressing the activation of the NF-κB signaling pathway

doi: 10.1371/journal.pone.0340382

Figure Lengend Snippet: Comparison of TTC staining between (A-B) sham group and MIRI group mice. (C-K) WB method was used to detect the expression ofTREM1and NF-κB signaling pathway related proteins p-IkBa, IkBa, p-NF-κB,NF-κB and the expression of pyroptosis related proteinsNLRP3、GSDMD-N、 Cleaved-Caspase-1, and mature IL-1β (IL-1β)、mature IL-18.ns indicates no statistical significance,*P < 0.05,**P < 0.01,****P < 0.0001.

Article Snippet: Primary antibody against NF-κB p65 (Abmart, T55034F) was added at a dilution of 1:100 and incubated at 4°C overnight.

Techniques: Comparison, Staining, Expressing

Journal: PLOS One

Article Title: Inhibition of TREM1 attenuates myocardial ischemia-reperfusion injury-induced cardiomyocyte pyroptosis by suppressing the activation of the NF-κB signaling pathway

doi: 10.1371/journal.pone.0340382

Figure Lengend Snippet: (A-C) Measure the cardiac function of mice after corresponding treatment measures using echocardiography. (D-E) H&E and immunohistochemistry was used to detect TREM1 expression in different groups. (scale bar: 25 μm). (F-G) Masson staining results of different groups. (scale bar: 100 μm).**P < 0.01,***P < 0.001,****P < 0.0001. Abbreviation: TREM-1, Triggering Receptor Expressed on Myeloid Cells-1; NF-κB, Nuclear Factor-κB; NLRP3, NOD-like Receptor Pyrin Domain-Containing Protein 3; H/R/HR, Hypoxia/Reoxygenation; MIRI, Myocardial Ischemia-Reperfusion Injury; I/R/IR, Ischemia-Reperfusion Injury.

Article Snippet: Primary antibody against NF-κB p65 (Abmart, T55034F) was added at a dilution of 1:100 and incubated at 4°C overnight.

Techniques: Immunohistochemistry, Expressing, Staining